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KMID : 0379119760040010053
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Korean Journal of Mycology
1976 Volume.4 No. 1 p.53 ~ p.58
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The Study of the Effect of Steroid to Fungal Growth iu vitro
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Abstract
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This study was undertaken to investigate the direct effect of three different kinds of steroid to Trichophyton(T.) mentagrophytes growth in vitro. A loopful of T. mentagrophytes was inoculated into Sabouraud´s media and Sabouraud´s media containing various concentrations of dexamethasone (0.04%-0.00004%) prednisolone (0.25%-0.00025%), triamcinolone acetonide (0.2%-0.0002%), Sabouraud´s media containing imidazole nitrate (econasole nitrate) 10 micro gm per ㏄. of media, and Sabouraud´s media containing econazole nitrate 10㎍ per ㏄. of media of various dexamethasone concentrations (0.04%-0.00004%). After inoculation they were left for two weeks at room temparature and the colony diameter were compared between Sabouraud´s media and Sabouraud´s media containing various concentrations of three different steroids, Sabouraud´s media containing econazole nitrate and Sabouraud´s media containing econazole nitrate of various dexamethasone concentrations.
For complementary study. hanging slide culture were done between Sabouraud´s media and Sabouraud´s media containing various dexamethasone concentrations to compare the degree of branching of hyphae and sporulation serially.
The study results were as follows:
1. There is no significant difference in growth rate of T. mentagrophytes between Sabouraud´s media and Sabouraud´s media containing varous concentrations of three different steroids.
2. There is no significant difference in growth rate of T. metagrophytes between Sabouraud´s media containing econozole nitrate 10 micro gm per cc. of media and Sabouraud´s media containing econozole nitrate 10 micro gm per cc. of media of various dexamethasone concentrations.
3. In hanging slide culture, there is no significant difference in branching of hyphac and sporulation between Sabouraud´s media and Sabouraud´s media containing various dexamethasone concentrations.
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